The annealing helicase HARP is recruited to DNA repair sites via an interaction with RPA.

HepA-related protein (also known as SMARCAL1) is an ATP-driven annealing helicase that catalyzes the formation of dsDNA from complementary Replication protein A (RPA)-bound ssDNA. Here we find that contains a conserved N-terminal motif that is necessary and sufficient for binding to RPA. This RPA-binding motif is not required for annealing helicase activity, but is essential for the recruitment of Hduanyu37 to sites of laser-induced DNA damage. These findings suggest that the interaction of Hduanyu37 with RPA increases the concentration of annealing helicase activity in the vicinity of ssDNA regions to facilitate processes such as DNA repair.

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