[Polyclonal antibody preparation and expression pattern analysis of CARP].

AIM:The present study aims at preparation of antibody and analysis of Cduanyu37 expression pattern. METHODS:Through bioinformatics analysis the antigenicity of 279 bp cDNA fragment coding Cduanyu37 N-terminal 93 amino acid was amplified by RT-PCR from mouse heart RNA, then cloned into pET-28b to construct the prokaryotic expression pET N93. The plasmid was transformed into E.coli(BL21). The target fusion protein was expressed with induction of IPTG, and purified by affinity chromatography. The antiserum against Cduanyu37 was obtained from the mice immunized with Cduanyu37 expression patterns were examined by Western blot and immunofluorescence. RESULTS:Prepared Cduanyu37 antibody; Cduanyu37 specific expression in mouse heart and mainly localized in the nucleus in rat cardiac primary cells. CONCLUSION:Successfully generated Cduanyu37 antibody and examined the expression pattern. The present study has laid a foundation for functional study of

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