Highly selective tuning of a silkworm olfactory receptor to a key mulberry leaf volatile.
[No authors listed]
摘要
BACKGROUND:The olfactory system plays an important role in the recognition of leaf volatiles during the search of folivore insects for a suitable plant host. For example, volatiles emitted by mulberry leaves trigger chemotaxis behavior in the silkworms Bombyx mori, and as a consequence, they preferentially reside on and consume mulberry leaves. Here, we aimed to identify natural chemoattractants and their corresponding olfactory receptors (Ors) involved in silkworm behavior to mulberry leaves. RESULTS:Chemotaxis behavioral assays for headspace volatiles detected by gas chromatography-mass spectroscopy analysis revealed that among the volatiles that were emitted by mulberry leaves, cis-jasmone was the most potent attractant for silkworms, working at a threshold of 30 pg from [corrected] 20 cm distance. Among a total of 66 Ors identified in the B. mori genome, we found that 23 were expressed in the olfactory organs during larval stages. Functional analysis of all the larvae-expressed Ors in Xenopus oocytes revealed that one Or, termed BmOr-56, showed a high sensitivity to cis-jasmone. In addition, the ligand-receptor activity of BmOr-56 reflected the chemotaxis behavioral response of silkworms. CONCLUSIONS:We identified cis-jasmone as a potent attractant in mulberry leaves for silkworms and provide evidence that a highly tuned receptor, BmOr-56, may mediate this behavioral attraction. The current study sheds light on the mechanism of the correlation between olfactory perception in folivore insects and chemotaxis behavior to a natural volatile emitted by green leaves.
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基因功能
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基因
Or-9, Or-33, Or-35, Or-37, Or-38, Or-45, Or-47, Or-14, Or-34, Or-25, Or-12, Or-36, Or-13, Or-21, Or-11, Or-20, Or-48P, Or-46, Or-24, Or-23, Or-32, Or-27, Or-29, Or-18, Or-16, Or-44, Or-39, Or-40, Or-7, Or-54, Or-60, Or-8, Or-17, Or-57, Or-59, Or-55, Or-22, Or-26, Or-28, Or-49, Or-50, Or-51, Or-52P, Or-53, Or-56, Or-58, Or-61, Or-62, Or-63, Or-64, Or-65, Or-66, Or-67, Or-68, Or-1, Or-3, Or-4, Or-5, Or-6, Or-2, Or-19, Or-30, Or-41, Or-15, Or-10, Or-42
原始数据
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文献解读
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