[Inhibitory effects of RNA interference on MMP-24 expression and invasiveness of ovarian cancer SKOV(3) cells].
[No authors listed]
摘要
OBJECTIVE:To investigate the inhibitory effect of RNA interference on MMP-24 expression and invasiveness of ovarian cancer SKOV(3) cells. METHOD:Two pairs of small interfering RNA (siRNA) specific to MMP-24 mRNA were designed and transfected into SKOV(3) cells. RT-PCR and Western blotting were used to detect the mRNA and protein expressions of MMP-24, and the cell invasiveness was assessed using an in vitro invasion test. RESULTS:After transfection with siRNA, the mRNA and protein expression levels of MMP-24 were obviously reduced in SKOV(3) cells, which also showed significantly decreased invasiveness in vitro. CONCLUSIONS:MMP-24 gene silencing by can suppress the invasiveness of ovarian cancer SKOV(3) cells in vitro, which may provide a new therapeutic approach of ovarian cancer.
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基因功能
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原始数据
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文献解读
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