例如:"lncRNA", "apoptosis", "WRKY"

A conserved small RNA promotes silencing of the outer membrane protein YbfM.

Mol. Microbiol.2009 May;72(3):566-77
Anders Aamann Rasmussen 1 , Jesper Johansen , Jesper S Nielsen , Martin Overgaard , Birgitte Kallipolitis , Poul Valentin-Hansen
Anders Aamann Rasmussen 1 , Jesper Johansen , Jesper S Nielsen , Martin Overgaard , Birgitte Kallipolitis , Poul Valentin-Hansen
+ et al

[No authors listed]

Author information
  • 1 Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, Odense M, Denmark.

摘要


In the past few years an increasing number of small non-coding RNAs (sRNAs) in enterobacteria have been found to negatively regulate the expression of outer membrane proteins (OMPs) at the post-transcriptional level. These RNAs act under various growth and stress conditions, suggesting that one important physiological role of regulatory RNA molecules in Gram-negative bacteria is to modulate the cell surface and/or to prevent accumulation of OMPs in the envelope. Here, we extend the OMP-sRNA network by showing that the expression of the OMP YbfM is silenced by a conserved sRNA, designated MicM (also known as RybC/SroB). The regulation is strictly dependent on the RNA chaperone Hfq, and mutational analysis indicates that MicM sequesters the ribosome binding site of ybfM mRNA by an antisense mechanism. Furthermore, we provide evidence that Hfq strongly enhances the on-rate of duplex formation between MicM and its target RNA in vitro, supporting the idea that a major cellular role of the RNA chaperone is to act as a catalyst in RNA-RNA duplex formation.