Phenotypic analysis of deflated/Ints7 function in Drosophila development.

The Drosophila gene deflated (CG18176; renamed after the pupal lethal abdominal phenotype of mutant individuals) is a member of a conserved gene family found in all multicellular organisms. The human orthologue of deflated (Ints7) encodes a subunit of the Integrator complex that associates with RNA polymerase II and has been implicated in snRNA processing. Since loss-of-function analyses of deflated have not yet been reported, we undertook to investigate deflated expression patterns and mutant phenotypes. deflated mRNA was detected at low levels in proliferating cells in postblastoderm embryos and GFP tagged protein is predominately nuclear. Generation and analysis of four mutant alleles revealed deflated is essential for normal development, as mutant individuals displayed pleiotropic defects affecting many stages of development, consistent with perturbation of cell signalling or cell proliferation. Our data demonstrate multiple roles in development for an Ints7 homologue and to demonstrate its requirement for normal cell signalling and proliferation.

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