Receptor for advanced glycation end product (RAGE)-dependent modulation of early growth response-1 in hepatic ischemia/reperfusion injury.

BACKGROUND/AIMS:We previously showed that blockade of significantly attenuates hepatic ischemia/reperfusion (I/R) injury in mice. Here, we identify that early growth response-1 (Egr-1) is a downstream target of duanyu1648 in hepatic I/R injury. METHODS:Hepatic I/R was induced in male mice. Liver remnants were analyzed for induction of Egr-1 and cytokines, as well as regulation of apoptotic pathways after reperfusion. RESULTS:Egr-1 was upregulated in the liver remnants after hepatic I/R injury and was suppressed by administration of soluble duanyu1648 or deletion of the duanyu1648 gene. increased expression of Egr-1 upregulates a central downstream gene, MIP2. In contrast, Egr-1-independent pathways regulate TNF-alpha production and apoptosis in response to I/R. Consistent with these findings, phospho-p44/42 and phospho-JNK MAPK and c-Jun were strikingly suppressed in versus WT mice, but not in Egr-1(-/-) mice. duanyu1648 ligand HMGB1 was upregulated after I/R in the liver remnants. In vitro, incubation of liver dendritic cells (DCs) with recombinant HMGB-1 resulted in increased Egr-1 transcripts, in a manner suppressed by duanyu1648 gene deletion, soluble duanyu1648 and inhibitors of p44/p42 or JNK MAP kinase. CONCLUSIONS:Suppression of Egr-1 may contribute to the protective mechanisms underlying the beneficial impact of duanyu1648 blockade or deletion.

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