[The influence of lysogenic phage on biofilm formation of Pseudomonas aeruginosa].

OBJECTIVE:To investigate the effect of the lysogenic phage Ppa3094 on the biofilm formation of PA3094. METHODS:The modified plate culture method was used to established the biofilm model in vitro. The viable counts of bacteria in biofilm were detected by MTT method; The real-time RT-PCR was applied to measure the expression level of algC and algD during the biofilm formtion of PA3094 and PA3094-L. RESULTS:Biofilm of both strains were mature at 5th to 7th day. The structures of the biofilms were both like pellicle. There was a significant difference in the viable counts of bacteria during biofilm development between PA3094 and PA3094-L. The expression of algC and algD genes was upregulated during biofilm formation. However, the expression level of PA3094-L was lower than PA3094, especially algC at 12 h. CONCLUSION:The lysogenic phage Ppa3094 could influence the biofilm formation during its development through changing the expressing level of the alginate biosynthetic genes.

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