DNA sampling: a method for probing protein binding at specific loci on bacterial chromosomes.
Nucleic Acids Res. 2009 Apr;37(5):e37. Epub 2009 Jan 30
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摘要
We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease, and is purified using FLAG-tagged LacI repressor and beads carrying anti-FLAG antibody. We illustrate the method by investigating the proteins bound to the colicin K gene regulatory region, either before or after induction of the colicin K gene promoter.
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基因功能
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原始数据
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文献解读
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