[No authors listed]
OBJECTIVE:To study the expression pattern of Polycomb gene Nspc1 at the early developmental stage in zebrafish. METHODS:In situ hybridization probe for Nspc1 was designed according to the GenBank information. Collecting zebrafish embryos at different stages including one cell stage, two-cell stage, bud stage, and somites stage, we hybridized them with the prepared probe. Then the hybridization signals at different intervals were observed and photographed at the right time. RESULTS:Nspc1 was expressed globally at the early stage. Its expression specificity began at the somites stage, mainly in the nervous system of the head. CONCLUSION:Nspc1 may play essential roles in the early stage development of zebrafish, especially in the nervous system.
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