High efficiency of ROS production by glycerophosphate dehydrogenase in mammalian mitochondria.

We investigated hydrogen peroxide production in mitochondria with low (liver, heart, brain) and high (brown adipose tissue, BAT) content of glycerophosphate dehydrogenase (mGPDH). production at state 4 due to electron backflow from mGPDH was low, but after inhibition of electron transport with antimycin A high rates of mGPDH-dependent duanyu1670 production were observed in liver, heart and brain mitochondria. When this duanyu1670 production was related to activity of mGPDH, many-fold higher duanyu1670 production was found in contrast to succinate- (39-, 28-, 3-fold) or pyruvate plus malate-dependent duanyu1670 production (32-, 96-, 5-fold). This specific rate of mGPDH-dependent duanyu1670 production was also exceedingly higher (28-, 66-, 22-fold) compared to that in BAT. mGPDH-dependent duanyu1670 production was localized to the dehydrogenase+CoQ and complex III, the latter being the highest in all mitochondria but BAT. Our results demonstrate high efficiency of mGPDH-dependent duanyu1670 production in mammalian mitochondria with a low content of mGPDH and suggest its endogenous inhibition in BAT.

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