Critical role for TARPs in early development despite broad functional redundancy.

Transmembrane AMPA receptor regulatory proteins including gamma-2, gamma-3, gamma-4, and gamma-8, are auxiliary subunits for AMPA receptors. Based on studies in single knockout mice, it has been suggested that nearly all native AMPA receptors are associated with To study the interplay between family members and AMPA receptors in vivo, we generated mice lacking multiple Tduanyu37s. Triple knockout mice lacking gamma-3, gamma-4, and gamma-8 are viable and fertile, and synaptic AMPA receptor activity is reduced to a level comparable to that seen in gamma-8 single knockout mice. In contrast, triple knockout mice lacking gamma-2, gamma-3, and either gamma-4 or gamma-8 cannot survive ex utero. In particular, gamma-2, gamma-3, gamma-4 triple knockout mice are born apneic and paralyzed, despite normal AMPA receptor function in cortical and spinal neurons. We found that gamma-8 is expressed at low levels in early postnatal mice and regulates AMPA receptor levels at this developmental time period. Thus, the early expression of gamma-8 may be responsible for maintaining AMPA receptor functions in neonatal neurons. Together, our data indicate that in particular gamma-2, are essential for early development, and that most neurons express multiple members of this functionally redundant protein family.

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