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Characterization of the Arabidopsis Brittle1 transport protein and impact of reduced activity on plant metabolism.

Plant J.2008 Oct;56(1):51-63. Epub 2008 Jun 28
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摘要


The Arabidopsis genome contains a gene (Atbt1) encoding a highly hydrophobic membrane protein of the mitochondrial carrier family, with six predicted transmembrane domains, and showing substantial structural similarity to Brittle1 proteins from maize and potato. We demonstrate that AtBT1 transports AMP, ADP and ATP (but not ADP-glucose), shows a unidirectional mode of transport, and locates to the plastidial membrane and not to the ER as previously proposed. Analysis using an Atbt1 promoter-GUS construct revealed substantial gene expression in rapidly growing root tips and maturating or germinating pollen. Survival of homozygous Atbt1::T-DNA mutants is very limited, and those that do survive produce non-fertile seeds. These observations indicate that no other carrier protein or metabolic mechanism can compensate for the loss of this transporter. Atbt1 dosage mutants show substantially retarded growth, adenylate levels similar to those of wild-type plants, increased glutamine contents and unchanged starch levels. Interestingly, the growth retardation of Atbt1 duanyu1615 mutant plants was circumvented by adenosine feeding, and was accompanied by increased adenylate levels. Further observations showed the presence of a functional nucleotide salvage pathway in Atbt1 duanyu1615 mutants. In summary, our data indicate that AtBT1 is a plastidial nucleotide uniport carrier protein that is strictly required to export newly synthesized adenylates into the cytosol.

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