[No authors listed]
The phylogenic alignment of homologous L35 protein suggests that human large subunit ribosomal protein L35 carries a 54 aa eukaryotic expansion segment (ES) at the C-terminal end. Within this ES, the first 25 amino acid residues were found to be essential for the nuclear import of the protein. The last 29 residues of the ES were shown to be uninvolved in the ribosome's structural and translational functions, although this region proved to be one of the contact sites for ribosomal docking to endoplasmic reticulum, as evident from the results of an in vivo recombinant ribosome analysis.
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