例如:"lncRNA", "apoptosis", "WRKY"

G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae.

Mol Biol Cell. 2008 Apr;19(4):1763-71. Epub 2008 Feb 06
Keiko Kono 1 , Satoru Nogami , Mitsuhiro Abe , Masafumi Nishizawa , Shinichi Morishita , David Pellman , Yoshikazu Ohya
Keiko Kono 1 , Satoru Nogami , Mitsuhiro Abe , Masafumi Nishizawa , Shinichi Morishita , David Pellman , Yoshikazu Ohya
+ et al

[No authors listed]

Author information
  • 1 Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba 277-8562, Japan.

摘要


Rho1p is an essential small GTPase that plays a key role in the morphogenesis of Saccharomyces cerevisiae. We show here that the activation of Rho1p is regulated by a cyclin-dependent kinase (CDK). Rho1p is activated at the G1/S transition at the incipient-bud sites by the Cln2p (G1 cyclin) and Cdc28p (CDK) complex, in a process mediated by Tus1p, a guanine nucleotide exchange factor for Rho1p. Tus1p interacts physically with Cln2p/Cdc28p and is phosphorylated in a Cln2p/Cdc28p-dependent manner. CDK phosphorylation consensus sites in Tus1p are required for both Cln2p-dependent activation of Rho1p and polarized organization of the actin cytoskeleton. We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition.