Resolving and identifying protein components of plant mitochondrial respiratory complexes using three dimensions of gel electrophoresis.
[No authors listed]
摘要
Analyzing highly hydrophobic proteins is a challenge for identification protocols based on gel separation and mass spectrometry. We combined Blue Native and 2D tricine gel electrophoresis to allow separation and identification of respiratory complex subunits from Arabidopsis mitochondria. We identified many of the highly hydrophobic mitochondrion-encoded subunits (GRAVY scores between +0.6 to +1.4) and also found a number of nucleus-encoded proteins associated with complex I for the first time in plants.
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基因功能
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基因
AT2G02050, AT2G02510, AT2G20360, PHB6, MGP1, AT2G27730, AT2G31490, ATP3, AT2G33220, AT2G40765, OEP16-3, AT2G42310, AT2G47690, AT3G06310, AT3G08610, AT3G10860, AT3G12260, MPPalpha, MPPBETA, AT3G03100, AT3G18410, AT3G27240, PHB4, AT3G46430, GAMMA CAL2, ATPQ, AT3G52730, AT3G57785, AT3G62790, AT4G16450, AT4G20150, AT4G00585, AT4G02580, PHB1, AT4G30010, AT4G32470, AT5G05370, CI51, AT5G08670, AT5G08680, AT5G08690, AT5G11770, AT5G13430, AT5G13440, ATP5, AT5G18800, AT5G25450, EMB1467, PHB3, AT5G40810, PHB7, AT5G47030, AT5G47570, AT5G47890, AT5G52840, AT5G59613, GAMMA CAL1, GAMMA CA3, FRO1, AT1G16700, GAMMA CA1, PHB2, MEE4, GAMMA CA2, AT1G49140, AT1G51650, AT1G51980, AT1G67350, AT1G67785, AT1G68680, AT1G76200, AT1G79010
原始数据
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文献解读
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