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Existence of an endogenous glutamate and aspartate transporter in Chinese hamster ovary cells.

Acta Biochim. Biophys. Sin. (Shanghai). 2007 Nov;39(11):851-6. doi:10.1111/j.1745-7270.2007.00354.x
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摘要


Chinese hamster ovary cells show endogenous high-affinity Na(+)-dependent glutamate transport activity. This transport activity is kinetically similar to a glutamate transporter family strategically expressed in the central nervous system and is pharmacologically unlike glutamate transporter-1 or excitatory amino acid carrier 1. The cDNA of a glutamate/aspartate transporter (GLAST)-like transporter was obtained and analyzed. The deduced amino acid sequence showed high similarity to human, mouse, and rat GLAST. We concluded that a GLAST-like glutamate transporter exists in Chinese hamster ovary cells that might confer the endogenous high-affinity Na(+)-dependent glutamate transport activity evident in these cells.

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