Screening of the osmotic pressure-inducible promoter regions from the whole genome of Escherichia coli by using a novel cloning method.

Biotechnol. Lett.2008 Apr;30(4):707-11. doi:10.1007/s10529-007-9583-3. Epub 2007 Nov 06

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Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressure-inducible promoter sequence was found. A mutation in this promoter sequence, introduced by site-specific mutagenesis, abolished its activity under the high osmotic pressure conditions. The method can thus be used for finding particular promoter sequences in the E. coli genome.

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Screening of the osmotic pressure-inducible promoter regions from the whole genome of Escherichia coli by using a novel cloning method.

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