例如:"lncRNA", "apoptosis", "WRKY"

Transcription factor E is a part of transcription elongation complexes.

J Biol Chem. 2007 Dec 07;282(49):35482-90. Epub 2007 Oct 05
Sebastian Grünberg 1 , Michael S Bartlett , Souad Naji , Michael Thomm
Sebastian Grünberg 1 , Michael S Bartlett , Souad Naji , Michael Thomm

[No authors listed]

Author information
  • 1 Lehrstuhl für Mikrobiologie, Universitaet Regensburg, 93053 Regensburg, Germany.

摘要


A homologue of the N-terminal domain of the alpha subunit of the general eukaryotic transcription factor TFE is encoded in the genomes of all sequenced archaea, but the position of archaeal TFE in transcription complexes has not yet been defined. We show here that TFE binds nonspecifically to single-stranded DNA, and photochemical cross-linking revealed TFE binding to a preformed open transcription bubble. In preinitiation complexes, the N-terminal part of TFE containing a winged helix-turn-helix motif is cross-linked specifically to DNA of the nontemplate DNA strand at positions -9 and -11. In complexes stalled at +20, TFE cross-linked specifically to positions +9, +11, and +16 of the non-template strand. Analyses of transcription complexes stalled at position +20 revealed a TFE-dependent increase of the resumption efficiency of stalled RNA polymerase and a TFE-induced enhanced permanganate sensitivity of thymine residues in the transcription bubble. These results demonstrate the presence of TFE in early elongation complexes and suggest a role of TFE in stabilization of the transcription bubble during elongation.