Molecular analysis of neurolysin expression in the rat and bovine ciliary body.
[No authors listed]
摘要
PURPOSE:This paper deals with the capability of the ciliary epithelium to express neurolysin, involved in the inactivation of numerous neuropeptides. METHODS:Total RNAs from ciliary body (CB) were processed for RT-PCR, and the amplification products were sequenced. The whole-protein extracts of CBs were analyzed using the Western blot. The CBs were processed for neurolysin immunolocalization. RESULTS:The RT-PCR detected the presence of neurolysin mRNA in the ciliary body. The Western blot assays demonstrated immunochemical cross-reactivity with neurolysin. The immunoreactivity to neurolysin was observed in ciliary epithelium. CONCLUSIONS:These results indicate that the ciliary epithelium expresses neurolysin.
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基因功能
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原始数据
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文献解读
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