Synthesis of nucleotide analogues by a promiscuous phosphoribosyltransferase.
Org. Lett.2007 Oct 11;9(21):4179-82. doi:10.1021/ol7016802. Epub 2007 Sep 14
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摘要
An Escherichia coli strain overexpressing a mutant variant of a phosphoribosyl transferase was developed as a catalyst for the efficient preparation of a range of purine nucleotide analogues. This system offers an efficient and rapid method for nucleotide analogue synthesis with 100% beta-selectivity, providing analytically pure product in a single purification step.
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文献解读
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