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Membrane topology of the GltS Na+/glutamate permease of Escherichia coli.

FEMS Microbiol. Lett.2007 Oct;275(1):71-9. Epub 2007 Jul 27
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摘要


The GltS Na+/glutamate permease of Escherichia coli is the most extensively studied member of the ESS family of bacterial glutamate:Na+ symporters. This paper presents the membrane topology analysis of the GltS with translational alkaline phosphatase and beta-galactosidase gene fusions generated by TnphoA, nested deletions and targeted fusions. The topology model suggested by the translational fusion technique is compared with the MemGen model and discussed in detail.

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