High-throughput analysis of spatio-temporal dynamics in Dictyostelium.
[No authors listed]
摘要
We demonstrate a time-lapse video approach that allows rapid examination of the spatio-temporal dynamics of Dictyostelium cell populations. Quantitative information was gathered by sampling life histories of more than 2,000 mutant clones from a large mutagenesis collection. Approximately 4% of the clonal lines showed a mutant phenotype at one stage. Many of these could be ordered by clustering into functional groups. The dataset allows one to search and retrieve movies on a gene-by-gene and phenotype-by-phenotype basis.
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基因功能
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基因
DDB_G0268266, dicB, gefV, acrA, mekA, gtaG, abpC, med23, yod1, lvsD, lvsB, DDB_G0273459, DDB_G0273515, DG1124, ggtA, abcA5, sfbA, DDB_G0274177, tipD, DDB_G0275747, DDB_G0276251, gefL, tipB, DDB_G0277165, piaA, clcD, DDB_G0278333, rsc12, DG1105, DDB_G0278945, DDB_G0279945, yelA, comH, dhkA, dstA, comE, tipA, DDB_G0281917, DDB_G0282093, DDB_G0282463, rdeA, lvsE, DDB_G0283015, migA, comD, splA, yakA, DDB_G0284177, gefA, regA, DDB_G0284619, DDB_G0285083, abcC9, gacT, dagA, phdA, tagB, ku80, erkA, prmt5, rgaA, DG1037, mkpA, roco5, DDB_G0288581, gbfA, DG1091, DDB_G0289377, usp12, DDB_G0290835, ctrA, adprt3, DDB_G0291926, DDB_G0292162, manA, comG, DG1098, DDB_G0293302, pakE
原始数据
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文献解读
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