例如:"lncRNA", "apoptosis", "WRKY"

ClpS modulates but is not essential for bacterial N-end rule degradation.

Genes Dev.2007 Feb 15;21(4):403-8
Kevin H Wang 1 , Robert T Sauer , Tania A Baker
Kevin H Wang 1 , Robert T Sauer , Tania A Baker

[No authors listed]

Author information
  • 1 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

摘要


In eubacteria, the ClpS adaptor has been proposed to be essential for degradation of N-end rule substrates by the AAA(+) protease ClpAP. To test this model, we assayed degradation of substrates bearing N-end rule sequences isolated in a genetic screen for efficient degradation tags. ClpS was not vital for degradation in vivo but rather stimulated turnover in a sequence-specific manner. Although ClpS substantially enhanced degradation of N-end substrates at low substrate concentrations in vitro, it suppressed the degradation rate when substrate was saturating. Thus, we conclude that ClpAP recognizes N-end rule substrates directly, whereas ClpS modulates this degradation pathway.

基因