Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.2006 Dec 01;62(Pt 12):1294-7. Epub 2006 Nov 30
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摘要
A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter-diffusion technique removes the disorder present in one dimension in crystals grown by either the batch or hanging-drop techniques. This makes the difference between useless crystals and crystals that allow successful determination of the structure of the protein. The crystals belong to space group P4, with unit-cell parameters a = b = 66.98, c = 427.09 A, and a data set was collected to 2.76 A.
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原始数据
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文献解读
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