Interaction of cytoskeleton genes with NSF2-induced neuromuscular junction overgrowth.

N-Ethylmaleimide sensitive factor (NSF) is an ATPase whose activity is important for intracellular trafficking. Previous genetic analysis of Drosophila NSF2 revealed a potential link between NSF and the actin cytoskeleton. The present study was therefore undertaken to specifically examine genetic interactions between the cytoskeleton and NSF. First, we tested for loss-of-function interaction and, indeed, we found that the combination of flies heterozygous for Act5C and NSF2 alleles led to reduced viability. Second, we expanded our gain-of-function approach to include cytoskeletal genes that were not included in our previous screen. Thirteen of 30 genes tested were found to suppress neuromuscular junction (NMJ) overgrowth. Altogether, these data support the idea that diverse NSF2 developmental and physiological phenotypes are related to disruption of the cytoskeleton and the large number of genes which can partially restore NMJ overgrowth and suggests that NSF may function near the top of the actin regulatory pathway.

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