Analysis of the C. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi.
[No authors listed]
摘要
Argonaute (AGO) proteins interact with small RNAs to mediate gene silencing. C. elegans contains 27 AGO genes, raising the question of what roles these genes play in and related gene-silencing pathways. Here we describe 31 deletion alleles representing all of the previously uncharacterized AGO genes. Analysis of single- and multiple-AGO mutant strains reveals functions in several pathways, including (1) chromosome segregation, (2) fertility, and (3) at least two separate steps in the duanyu1615 pathway. We show that RDE-1 interacts with trigger-derived sense and antisense RNAs to initiate while several other AGO proteins interact with amplified siRNAs to mediate downstream silencing. Overexpression of downstream AGOs enhances silencing, suggesting that these proteins are limiting for Interestingly, these AGO proteins lack key residues required for mRNA cleavage. Our findings support a two-step model for duanyu1615, in which functionally and structurally distinct AGOs act sequentially to direct gene silencing.
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基因功能
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原始数据
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文献解读
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