Characterization of the aldo-keto reductase 1C gene cluster on pig chromosome 10: possible associations with reproductive traits.

BACKGROUND:The rate of pubertal development and weaning to estrus interval are correlated and affect reproductive efficiency of swine. Quantitative trait loci (QTL) for age of puberty, nipple number and ovulation rate have been identified in Meishan crosses on pig chromosome 10q (SSC10) near the telomere, which is homologous to human chromosome 10p15 and contains an aldo-keto reductase (AKR) gene cluster with at least six family members. AKRs are tissue-specific hydroxysteroid dehydrogenases that interconvert weak steroid hormones to their more potent counterparts and regulate processes involved in development, homeostasis and reproduction. Because of their location in the swine genome and their implication in reproductive physiology, this gene cluster was characterized and evaluated for effects on reproductive traits in swine. RESULTS:Screening the porcine CHORI-242 BAC library with a full-length AKR1C4 cDNA identified 7 positive clones and sample sequencing of 5 BAC clones revealed 5 distinct AKR1C genes (AKR1CL2 and AKR1C1 through 4), which mapped to 126-128 cM on SSC10. Using the IMpRH7000rad and IMNpRH212000rad radiation hybrid panels, these 5 genes mapped between microsatellite markers SWR67 and SW2067. Comparison of sequence data with the porcine BAC fingerprint map show that the cluster of genes resides in a 300 kb region. Twelve SNPs were genotyped in gilts observed for age at first estrus and ovulation rate from the F8 and F10 generations of one-quarter Meishan descendants of the USMARC resource population. Age at puberty, nipple number and ovulation rate data were analyzed for association with genotypes by MTDFREML using an animal model. One SNP, a phenylalanine to isoleucine substitution in AKR1C2, was associated with age of puberty (p = 0.07) and possibly ovulation rate (p = 0.102). Two SNP in AKR1C4 were significantly associated with nipple number (p (1)unit from which nucleic acids are constructed by polymerization. It contains a sugar, a phosphate group, and an organic base. ATP is a nucleotide.
(2)the unit of DNA, consisting of one base, one phosphate molecule, and the sugar deoxyribose. See also base/base pair
(3)organic molecules that serve as the monomers, or subunits, of nucleic acids like DNA and RNA. They include adenine (A), cytosine (C), guanine (G), thymine (T), and uracil (U).
(4)One of the structural components, or building blocks, of DNA and RNA. A nucleotide consists of a base (one of four chemicals: adenine, thymine [uracil instead of thymine for RNA], guanine, and cytosine) plus a molecule of sugar [ribose for RNA, deoxyribose for DNA] and one of phosphoric acid (from the National Human Genome Research Institute (NHGRI) Glossary of Genetic Terms {http://www.genome.gov/glossary.cfm})
(5)Chain of molecules which make up the structural units of DNA and RNA. Nucleotides comprise a sugar, nitrogenous base and phosphate group.
(6)A unit building block of DNA and RNA. A nucleotide consists of a sugar and phosphate backbone with a base attached.
(7)A monomer unit of nucleic acid, consisting of a purine or pyrimidine base, a sugar molecule (ribose or deoxyribose), and phosphate group(s).
(8)A molecule consisting of a nitrogenous base (A,G, T, or C in DNA; A, G, U or C in RNA), a phosphate moiety and a sugar group (deoxyribose in DNA and ribose in RNA). Thousands of nucleotides are linked to form a DNA or RNA molecule.

" data-original-title="Nucleotide" data-html="true" data-trigger="foucs">nucleotide sequence data reported have been submitted to GenBank and assigned accession numbers [GenBank:DQ474064-DQ474068, GenBank:DQ494488-DQ494490 and GenBank:DQ487182-DQ487184].

KEYWORDS: {{ getKeywords(articleDetailText.words) }}