Zebrafish promoter microarrays identify actively transcribed embryonic genes.
Genome Biol. 2006;7(8):R71. Epub 2006 Aug 04
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摘要
We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.
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基因功能
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原始数据
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文献解读
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