Crystallization of quinoprotein glucose dehydrogenase variants and homologues by microseeding.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.2006 Jun 01;62(Pt 6):518-21. Epub 2006 May 05
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摘要
The soluble quinoprotein glucose dehydrogenase oxidizes glucose, maltose and a variety of other monosaccharides and disaccharides to the corresponding lactones. An efficient microseeding protocol is reported to produce crystals of three variants that display reduced activity towards maltose. Similar cross-seeding protocols to grow crystals of homologues from Escherichia coli and Streptomyces coelicolor are described.
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文献解读
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