ATP-binding cassette transporters are required for efficient RNA interference in Caenorhabditis elegans.

RNA interference is a conserved gene-silencing phenomenon that can be triggered by delivery of double-stranded RNA (dsRNA) to cells and is a widely exploited technology in analyses of gene function. Although a number of proteins that facilitate have been identified, current descriptions of duanyu1615 and interrelated mechanisms are far from complete. Here, we report that the Caenorhabditis elegans gene haf-6 is required for efficient HAF-6 is a member of the ATP-binding cassette (ABC) transporter gene superfamily. ABC transporters use ATP to translocate small molecule substrates across the membranes in which they reside, often against a steep concentration gradient. Collectively, ABC transporters are involved in a variety of activities, including protective or barrier mechanisms that export drugs or toxins from cells, organellar biogenesis, and mechanisms that protect against viral infection. HAF-6 is expressed predominantly in the intestine and germline and is localized to intracellular reticular organelles. We further demonstrate that eight additional ABC genes from diverse subfamilies are each required for efficient duanyu1615 in C. elegans. Thus, the ability to mount a robust duanyu1615 response to dsRNA depends upon the deployment of two ancient systems that respond to environmental assaults: duanyu1615 mechanisms and membrane transport systems that use ABC proteins.

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