Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution.

Biotechnol. Bioeng.2006 Apr 5;93(5):1023-7. doi:10.1002/bit.20754

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摘要

D-amino acid oxidase (DAO) is of considerable practical importance, such as bioconversion and enzymatic assay. In this study, we succeeded in obtaining a thermostable mutant DAO from porcine kidney by a single amino acid substitution. This mutant enzyme, F42C, was stable at 55 degrees C, while the wild-type enzyme was stable only up to 45 degrees C. The Km values of F42C for D-amino acids was about half of those of the wild-type enzyme. This mutant DAO with improved stability and affinity for its substrates is advantageous for the determination of D-amino acids.

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Thermostabilization of porcine kidney D-amino acid oxidase by a single amino acid substitution.

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