Artificial polypeptide scaffold for protein immobilization.

J. Am. Chem. Soc.2005 Jul 27;127(29):10136-7. doi:10.1021/ja051457h

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An artificial polypeptide scaffold composed of surface anchor and protein capture domains was designed and expressed in vivo. By using a mutant E. coli phenylalanyl-tRNA synthetase, the photoreactive amino acid para-azidophenylalanine was incorporated into the surface anchor domain. Octyltrichlorosilane-treated surfaces were functionalized with this polypeptide by spin coating and photocrosslinking. The resulting protein films were shown to immobilize recombinant proteins through association of coiled coil heterodimer.

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Artificial polypeptide scaffold for protein immobilization.

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