Cloning and expression of bovine sodium/glucose cotransporter SGLT2.

The second member of the Na(+)/glucose cotransporter family, SGLT2, is a low-affinity active glucose transporter. In humans, it is predominantly located on the apical membrane of the S1 and S2 segments of renal proximal convoluted tubules, and, thus, may be mainly responsible for the reabsorption of D-glucose from the glomerular filtrate. By BLAST searching the GenBank database, we found expressed sequence tag sequences of SGLT2 in the cDNA library of bovine mammary tissues, indicating its expression in bovine mammary gland. To facilitate study of the mechanism of glucose reabsorption in bovine kidneys in maintenance of glucose homeostasis of lactating cows and the potential role of SGLT2 in the mammary gland, we cloned bovine SGLT2 and examined the distribution of its mRNA expression in bovine tissues. The full length mRNA of bSGLT2 is 2275 bp, and is predicted to encode a protein of 673 amino acids, with a molecular weight of approximately 73 kDa. The deduced amino acid sequence of bovine SGLT2 is 91, 90, 91, and 90% identical to human, rabbit, mouse, and rat SGLT2, respectively, and is 58 and 48% identical to bovine SGLT1 and SGLT5, respectively. The sequence of bSGLT2 contains several characteristically conserved sodium:solute symporter family signatures. Analysis of current bovine genomic data indicates that the bovine SGLT2 gene may consist of 14 exons. The major in vitro transcription and translation product of bovine SGLT2 cDNA migrated at an apparent molecular weight of 55 kDa. The SGLT2 mRNA was detected predominantly in bovine kidney as a 2.3-kb transcript, and at lower levels in all other bovine tissues examined, including the mammary gland, liver, lung, spleen, intestine, and skeletal muscle, as a 3.0-kb transcript. Expression of SGLT2 mRNA in bovine mammary gland increased more than 10-fold from late pregnancy to early lactation, similar to SGLT1. This indicates that SGLT2 may play a role in milk synthesis in the lactating mammary gland.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}