A scalable, GFP-based pipeline for membrane protein overexpression screening and purification.

Protein Sci. 2005 Aug;14(8):2011-7. Epub 2005 Jun 29

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We describe a generic, GFP-based pipeline for membrane protein overexpression and purification in Escherichia coli. We exemplify the use of the pipeline by the identification and characterization of E. coli YedZ, a new, membrane-integral flavocytochrome. The approach is scalable and suitable for high-throughput applications. The GFP-based pipeline will facilitate the characterization of the E. coli membrane proteome and serves as an important reference for the characterization of other membrane proteomes.

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A scalable, GFP-based pipeline for membrane protein overexpression screening and purification.

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