[Mapping of regulatory domain of T-protein from Escherichia coli].

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2005 Mar;34(2):181-4. doi:10.3785/j.issn.1008-9292.2005.02.018

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OBJECTIVE:To map the regulatory domain of Escherichia coli T-protein. METHODS:Fragmentation cloning was employed in cloning of 11 fragments from T-protein. The regulatory activity of each fragment was determined respectively. RESULTS:The regulatory domain of T-protein was located in the C-terminal 270 amino acids, which was the same location as PDH domain. CONCLUSION:T-protein has no independent regulatory domain.

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[Mapping of regulatory domain of T-protein from Escherichia coli].

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