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Delta-interacting protein A, a new inhibitory partner of CCAAT/enhancer-binding protein beta, implicated in adipocyte differentiation.

J Biol Chem. 2005 Mar 25;280(12):11432-8. Epub 2005 Jan 11
Olivier Bezy 1 , Christian Elabd , Olivia Cochet , Rasmus K Petersen , Karsten Kristiansen , Christian Dani , Gérard Ailhaud , Ez-Zoubir Amri
Olivier Bezy 1 , Christian Elabd , Olivia Cochet , Rasmus K Petersen , Karsten Kristiansen , Christian Dani , Gérard Ailhaud , Ez-Zoubir Amri
+ et al

[No authors listed]

Author information
  • 1 Institute of Signaling, Developmental Biology, and Cancer Research, Centre de Biochimie, UMR 6543 CNRS, Faculté des Sciences, Parc Valrose, 06108 Nice cedex 2, France.

摘要


CCAAT/enhancer-binding protein beta (C/EBP beta) is expressed early during the adipocyte differentiation program and plays an important role in this process. In an attempt to identify novel proteins that interact with C/EBP beta, we performed a yeast two-hybrid screen with a preadipocyte cDNA library and identified a new co-regulator, delta-interacting protein A (DIPA). DIPA mRNA is expressed during adipocyte differentiation of clonal cell lines. DIPA interacts with C/EBP beta and -delta proteins in intact cells and inhibits their transcriptional activity but not that of C/EBP alpha. Stable overexpression of DIPA in preadipocytes partially inhibits adipocyte differentiation, whereas its gene silencing enhances this process. DIPA and C/EBP beta co-localize in the nucleus, and overexpression of DIPA in preadipocytes results in a partial inhibition of the mitotic clonal expansion which is critical for differentiation. Thus, DIPA is a novel partner of C/EBP beta that down-regulates early events of adipogenesis.