Dihydroorotate dehydrogenase mRNA and protein expression analysis in normal and drug-resistant cells.
Nucleosides Nucleotides Nucleic Acids. 2004 Oct;23(8-9):1281-5. doi:10.1081/NCN-200027547
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摘要
To follow the expression of the fourth enzyme of pyrimidine de novo synthesis dihydroorotate dehydrogenase (DHODH) in cells and tissues, we studied the DHODH mRNA expression by means of RT-PCR in rat tissues. Rabbit polyclonal anti-DHODH immunoglobulins were applied for immunochemical quantification of the enzyme protein by Western blotting. In mouse B-lymphocytes, which were adapted to tolerate up to a 50-fold concentration of the DHODH inhibitor leflunomide, a 20 fold protein overexpression was measured. Southern blotting indicated DHODH gene amplification.
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基因功能
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原始数据
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文献解读
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