Identification of new modes of Dd-STATa regulation of gene expression in Dictyostelium by in situ hybridisation.

Signal Transducers and Activators of Transcription are transcription factors which lie at the end of cytokine and growth signal transduction pathways. Dictyostelium is a functional homologue of metazoan It is activated by cAMP and, at the slug stage, it translocates into the nuclei of the tip cells, which are a subset of the anterior, prestalk A (pstA) cells. Here we searched for novel Dd-duanyu1813a regulated genes by in situ hybridisation. A set of 54 cDNA clones whose gene expression patterns are known to be prestalk-specific (Maeda et al., 2003), were chosen as probes and we compared their expression patterns in parental and strains. We identified 13 genes which are candidates for direct induction by In the parental strain, most of these genes are expressed in the cone shaped mass of pstAB cells which is located within the prestalk region. These cDNAs show little or no expression in the Dd-duanyu1813a-null strain. This contrasts markedly with the paradigmatic ecmB gene which is expressed in pstAB cells in parental cells, but which is expressed throughout the prestalk zone in the Dd-duanyu1813a-null strain. We also identified several genes which are normally expressed in pstA cells, or throughout the prestalk region, but whose expression is markedly down-regulated in the null mutant. Again, this contrasts with markers derived from the paradigmatic, ecmA gene which are expressed normally in the Dd-duanyu1813a-null strain. The identification of these novel genes provides valuable tools to investigate the role of

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