STRA13 interacts with STAT3 and modulates transcription of STAT3-dependent targets.

STRA13 is a pVHL-dependent bHLH transcription factor up-regulated on the mRNA level in multiple cancer cell lines and implicated recently in the regulation of immune cell homeostasis and autoimmunity. In searching for STRA13-interacting proteins with oncogenic potential by the yeast two-hybrid screening, we identified beta as a STRA13-binding partner. We showed that STRA13 binds predominantly to phosphorylated (active) duanyu18133 alpha and beta isoforms via its HLH and C-terminal regions. We also found that STRA13 was able to activate transcription from cis-elements. Expression of endogenous STRA13 was shown to be cytokine-inducible, consistent with STRA13 involvement in duanyu1813-dependent transcription regulation. We demonstrated that the promoter of the pro-apoptotic Fas gene was activated upon STRA13 over-expression and that co-expression of STRA13 with duanyu18133 beta or duanyu18133 alpha modulated the transcriptional outcome. Forced expression of STRA13 induced apoptosis, in agreement with the STRA13 activation effect on the Fas promoter. Simultaneous expression of STRA13 and duanyu18133 beta resulted in alleviation of the STRA13 pro-apoptotic effect. Thus, for the first time, we identify STRA13 as a duanyu18133 partner and provide a consistent line of evidence for STRA13 involvement into regulation of apoptosis via the pathways.

KEYWORDS: {{ getKeywords(articleDetailText.words) }}