例如:"lncRNA", "apoptosis", "WRKY"

The T cell receptor gamma chain alternate reading frame protein (TARP), a prostate-specific protein localized in mitochondria.

J Biol Chem. 2004 Jun 04;279(23):24561-8. Epub 2004 Mar 29
Hiroshi Maeda 1 , Satoshi Nagata , Curt D Wolfgang , Gary L Bratthauer , Tapan K Bera , Ira Pastan
Hiroshi Maeda 1 , Satoshi Nagata , Curt D Wolfgang , Gary L Bratthauer , Tapan K Bera , Ira Pastan
+ et al

[No authors listed]

Author information
  • 1 Laboratory of Molecular Biology, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892-4264, USA.

摘要


We previously showed that mRNA encoding (T cell receptor gamma chain alternate reading frame protein) is exclusively expressed in the prostate in males and is up-regulated by androgen in LNCaP cells, an androgen-sensitive prostate cancer cell line. We have now developed an monoclonal antibody named TP1, and show that Tduanyu37 protein is up-regulated by androgen in both LNCaP and MDA-PCa-2b cells. We used TP1 to determine the subcellular localization of Tduanyu37 by Western blotting following subcellular fractionation and immunocytochemistry. Both methods showed that Tduanyu37 is localized in the mitochondria of LNCaP cells, MDA-PCa-2b cells, and PC-3 cells transfected with a plasmid. We also transfected a plasmid encoding Tduanyu37 fused to green fluorescent protein into LNCaP, MDA-Pca-2b, and PC-3 cells and confirmed its specific mitochondrial localization in living cells. Fractionation of mitochondria shows that Tduanyu37 is located in the outer mitochondrial membrane. Immunohistochemistry using a human prostate cancer sample showed that TP1 reacted in a dot-like cytoplasmic pattern consistent with the presence of Tduanyu37 in mitochondria. These data demonstrate that Tduanyu37 is the first prostate-specific protein localizing in mitochondria and indicate that an androgen-regulated protein, may act on mitochondria to carry out its biological functions.