Left-right asymmetric ventricular expression of CARP in the piglet heart: regional response to experimental heart failure.

BACKGROUND AND AIM:Cardiac ankyrin repeat protein whose expression is down-regulated in response to doxorubicin (Dox) in vitro, has been proposed to be a marker of experimentally-induced cardiac hypertrophy in rodent models. In piglets, the rapid hypertrophy rate of the left ventricle (LV) as compared to that of the right ventricle (RV) represents a natural model of asymmetric ventricular enlargement. We tested whether expression correlates with postnatal ventricular hypertrophy and to what extent Cduanyu37 can be sensitive to Dox treatment in mRNA and protein levels were quantified (by Northern blot hybridization, semi-quantitative RT-PCR and Western blot) in the piglet heart, both during early postnatal development and upon Dox-induced cardiomyopathy (Dox-CM). RESULTS:The study revealed: (1) significantly augmented Cduanyu37 mRNA and protein levels in the LV compared to the RV resulting in left vs. right asymmetry in ventricular Cduanyu37 expression throughout early postnatal development; (2) dose- and chamber-dependent Cduanyu37 mRNA and protein enrichment in ventricular myocardium in response to Dox; and (3) abolishment of asymmetric patterns of ventricular Cduanyu37 expression at heart failure resulting from Dox-CM. CONCLUSIONS:(1) Cduanyu37 is differentially regulated in the LV and RV during both postnatal development and disease; and (2) monitoring of ventricular Cduanyu37 expression patterns can be used for further analysis of transition from compensated to overt heart failure.

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