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The cDNA RPE1 and monoclonal antibody HMB-50 define gene products preferentially expressed in retinal pigment epithelium.

Exp. Eye Res.1992 Nov;55(5):657-62. doi:10.1016/0014-4835(92)90170-w
R Y Kim 1 , G J Wistow
R Y Kim 1 , G J Wistow

[No authors listed]

Author information
  • 1 Laboratory of Molecular and Developmental Biology, National Eye Institute, National Institutes of Health, Bethesda, MD 20892.

摘要


As a first step towards identifying genes expressed specifically in the retinal pigment epithelium (RPE), we generated a bovine RPE cDNA library. In preliminary characterization of this library, we isolated a clone, RPE1, that was identified as the bovine homologue of a human cDNA thought to encode the HMB-50 melanoma antigen. Sequence analysis suggests RPE1 encodes a membrane-anchored glycoprotein. In Northern blot analysis of RNA from bovine tissues, the 2.5 kb RPE1 transcript was detected only in the RPE. Southern blot analysis suggested the presence of a single bovine rpe1 gene. In normal human eyes, HMB-50 immunoreactivity was also restricted to the RPE; no immunoreactivity was detected in other intraocular pigmented cells. Thus, RPE1 and HMB-50 define gene products preferentially expressed in the RPE at the RNA and protein levels, respectively.