An efficient enzymatic synthesis of thiamin pyrophosphate.

Bioorg. Med. Chem. Lett.2003 Nov 17;13(22):4139-41

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Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.

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An efficient enzymatic synthesis of thiamin pyrophosphate.

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