Identification of plu genes and cis-acting elements of PCNA in the Drosophila genus using conservation of gene order.

In Drosophila melanogaster, the cell cycle control gene, plutonium (plu), is located between the PCNA and RpS18 genes at position 56F on chromosome arm 2R. We have used a comparative genomic approach to investigate the evolution of the plu gene and to locate conserved cis-acting elements for plu, RpS18 and PCNA. Using primers within coding regions of PCNA and RpS18, we amplified and sequenced the intervening region from twelve Drosophila species. In each species, this region contains a plu gene resembling the D. melanogaster gene in size and in the number and position of introns. The predicted Plu sequence from the different species demonstrates that the first two ankyrin repeats are conserved. Of the transcriptional control elements of D. melanogaster PCNA, we found that three motifs 5' to the PCNA transcription unit are conserved in Drosophila species.

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