Phospholipid scramblase 3 is the mitochondrial target of protein kinase C delta-induced apoptosis.

Protein kinase C delta translocates to mitochondria during apo-ptosis,but its mitochondrial target remains unclear. We found that physically interacted with and phosphorylated phospholipid scramblase 3 (PLS3) after UV irradiation. PLS3 is a high-affinity substrate for duanyu1531-delta in vitro with the K(m) at 10.5 nM. Cells expressing wild-type PLS3 became apoptotic on phorbol ester stimulation, whereas the control cells did not. Expression of a mitochondrial-targeted duanyu1531-delta enhanced apoptosis more prominently in HeLa-PLS3 cells than control HeLa cells and HeLa cells expressing an inactive PLS3 mutant. These results indicate that PLS3 is a downstream effector of duanyu1531-delta in the mitochondria.

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