Inducible RNA interference uncovers the Drosophila protein Bx42 as an essential nuclear cofactor involved in Notch signal transduction.

We used the UAS/GAL4 two component system to induce mRNA interference during Drosophila development. In the adult eye the expression from white transgenes or the resident white locus is significantly repressed by the induction of using different GAL4 expressing strains. By induced we demonstrate that the conserved nuclear protein Bx42 is essential for the development of many tissues. Phenotypically the effects of Bx42 duanyu1615 resemble those obtained for certain classes of Notch mutants, pointing to an involvement of Bx42 in the Notch signal transduction pathway. The wing phenotype following overexpression of Suppressor of Hairless is strongly enhanced by simultaneous Bx42 duanyu1615 induction in the same tissue. Target genes of Notch signaling like cut and Enhancer of split m8 were suppressed by induction of Bx42 Our results demonstrate that inducible duanyu1615 is a powerful tool to study the role of essential genes throughout development.

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