RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E.

Gene silencing by double-stranded RNA is a widespread phenomenon called involving homology-dependent degradation of mRNAs. Here we show that is established in the Drosophila female germ line. mRNA transcripts are translationally quiescent at the arrested oocyte stage and are insensitive to Upon oocyte maturation, transcripts that are translated become sensitive to degradation while untranslated transcripts remain resistant. Mutations in aubergine and spindle-E, members of the PIWI/PAZ and DE-H helicase gene families, respectively, block duanyu1615 activation during egg maturation and perturb translation control during oogenesis, supporting a connection between gene silencing and translation in the oocyte.

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