Lack of regulation of the modification-dependent restriction enzyme McrBC in Escherichia coli.

J Bacteriol. 2002 Mar;184(6):1794-5. doi:10.1128/JB.184.6.1794-1795.2002

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摘要

Restriction alleviation (RA) by the type I restriction enzyme EcoKI is caused by treatments that damage DNA. RA is due to proteolysis of the EcoKI HsdR subunit by the ClpXP ATP-dependent protease. Here we show that the modification-dependent enzyme McrBC is not subject to RA, although it is moderately sensitive to ClpAP.

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Lack of regulation of the modification-dependent restriction enzyme McrBC in Escherichia coli.

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