Cloning of porcine IGF1 receptor cDNA and detection of sequence polymorphisms using RT-PCR.

Anim. Genet.2001 Dec;32(6):386-9

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A cDNA encoding the porcine type 1 insulin-like growth factor receptor (IGF1R) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR). The sequence of a 4.2-kb product was determined and had an open reading frame, encoding 1367 amino acids with 98.1 and 95.2% sequence similarity to the human and rat IGF1R, respectively. In the comparison of RT-PCR derived IGF1R sequences from 12 unrelated pigs, 12 silent sequence variants were found.

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Cloning of porcine IGF1 receptor cDNA and detection of sequence polymorphisms using RT-PCR.

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